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COL2A1 Rabbit pAb

Anti-COL2A1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216437

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216437
Product Name:   Anti-COL2A1 Rabbit Polyclonal Antibody
Synonyms:   AOM; ANFH; SEDC; STL1; COL11A3
UNIPROT ID:   P02458 (Gene Accession – BC007252 )
Background:    This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.
Immunogen:    Fusion protein of human COL2A1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Stem Cells
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 216437(COL2A1 Antibody) at a dilution of 1/60(Secreted).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 216437(Anti-COL2A1 Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 216437(Anti-COL2A1 Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with fusion protein and then with D220453(Anti-COL2A1 Antibody) at dilution 1/60.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: C2C12 cell, Rat skeletal muscle tissue lysates;
Primary antibody: 216437(COL2A1 Antibody) at dilution 1/700;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 minutes
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