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CORO2B Rabbit pAb

Anti-CORO2B Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221818

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221818
Product Name:   Anti-CORO2B Rabbit Polyclonal Antibody
Synonyms:   CLIPINC
UNIPROT ID:   Q9UQ03 (Gene Accession – NP_006082 )
Background:    Coronins are a family of WD repeat-containing, actin-binding proteins that localize to submembraneous areas and regulate cell motility and cytoskeletal rearrangement. Coronin 1A (CORO1A, CLIPINA, CLABP, TACO, p57) can form coiled coil-mediated homotrimeric complexes that influence early phagosome formation. PKC-dependent phosphorylation of Coronin 1B (CORO1B) at Serine 2 regulates leading edge dynamics and cell motility in fibroblasts through interactions with Arp2/3 complex. Coronin 1C (CORO1C, Coronin 3, HCRNN4) is abundant in differentiating Neuro-2a cells, PC-12 cells and primary oligodendrocytes, where it is thought to influence neuron morphogenesis and migration. Coronin 2A (CORO2A, CLIPINB, IR10, WDR2) is a component of the approximately 1.5-2 megadalton N-CoR (nuclear receptor corepressor) complex of 10-12 proteins, which recruits HDACs to generate repressive chromatin. Coronin 7 (CORO7, CRN7) localizes to the Golgi membrane and influences the organization of intracellular membrane compartments and vesicular trafficking. Coronin 2B (CORO2B, CLIPINC) and Coronin 6 (CORO6) are similar to other members of this family, since they possess a conserved basic N-terminal motif and 3-10 WD repeats clustered in one to two core domains.
Immunogen:    Synthetic peptide of human CORO2B
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 40-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221818(CORO2B Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221818(Anti-CORO2B Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Mouse brain tissue, NIH/3T3 cell lysates;
Primary antibody: 221818(CORO2B Antibody) at dilution 1/1000;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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