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COX4I1 Rabbit pAb

Anti-COX4I1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221194

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221194
Product Name:   Anti-COX4I1 Rabbit Polyclonal Antibody
Synonyms:   COX4; COXIV; COX4-1; COXIV-1; MC4DN16; COX IV-1
UNIPROT ID:   P13073 (Gene Accession – NP_001852 )
Background:    Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3′ of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms.
Immunogen:    Synthetic peptide of human COX4I1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human gastric cancer tissue using 221194(COX4I1 Antibody) at a dilution of 1/25(Cytoplasm).

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using 221194(Anti-COX4I1 Antibody) at a dilution of 1/25.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: A549, PC3, 293T, NIH/3T3, Hela, HepG2, Mouse skeletal muscle tissue lysates;
Primary antibody: 221194(COX4I1 Antibody) at dilution 1/500;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds
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