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COX6B1 Rabbit pAb

Anti-COX6B1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216278

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216278
Product Name:   Anti-COX6B1 Rabbit Polyclonal Antibody
Synonyms:   COXG; COX6B; MC4DN7; COXVIb1
UNIPROT ID:   P14854 (Gene Accession – BC001015 )
Background:    Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes subunit VIb. Mutations in this gene are associated with severe infantile encephalomyopathy. Three pseudogenes COX6BP-1, COX6BP-2 and COX6BP-3 have been found on chromosomes 7, 17 and 22q13.1-13.2, respectively.
Immunogen:    Fusion protein of human COX6B1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 216278(COX6B1 Antibody) at a dilution of 1/95(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the fusion protein and then with 216278(Anti-COX6B1 Antibody) at dilution 1/95.

The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 216278(Anti-COX6B1 Antibody) at a dilution of 1/95.

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with fusion protein and then with D220116(Anti-COX6B1 Antibody) at dilution 1/95.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: LOVO and HepG2 cell lysates;
Primary antibody: 216278(COX6B1 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes
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