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COX7A2 Rabbit pAb

Anti-COX7A2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216109

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216109
Product Name:   Anti-COX7A2 Rabbit Polyclonal Antibody
Synonyms:   VIIAL; COX7AL; COX7AL1; COXVIIAL; COXVIIa-L
UNIPROT ID:   P14406 (Gene Accession – NP_001856 )
Background:    Cytochrome c oxidase, the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of three catalytic subunits encoded by mitochondrial genes, and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, while the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (liver isoform) of subunit VIIa, with this polypeptide being present in both muscle and non-muscle tissues. In addition to polypeptide 2, subunit VIIa includes polypeptide 1 (muscle isoform), which is present only in muscle tissues, and a related protein, which is present in all tissues. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 4 and 14.
Immunogen:    Synthetic peptide of human COX7A2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-50;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 216109(COX7A2 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 216109(Anti-COX7A2 Antibody) at dilution 1/20.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Hela cell lysate;
Primary antibody: 216109(COX7A2 Antibody) at dilution 1/500;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
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