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CXCR3 Rabbit pAb

Anti-CXCR3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216456

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216456
Product Name:   Anti-CXCR3 Rabbit Polyclonal Antibody
Synonyms:   GPR9; MigR; CD182; CD183; Mig-R; CKR-L2; CMKAR3; IP10-R
UNIPROT ID:   P49682 (Gene Accession – BC034403 )
Background:    This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716).
Immunogen:    Fusion protein of human CXCR3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cardiovascular, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 216456(CXCR3 Antibody) at a dilution of 1/70(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 216456(Anti-CXCR3 Antibody) at dilution 1/70.

The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 216456(Anti-CXCR3 Antibody) at a dilution of 1/70.

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with fusion protein and then with D220505(Anti-CXCR3 Antibody) at dilution 1/70.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: LO2 cell lysate ;
Primary antibody: 216456(CXCR3 Antibody) at dilution 1/700;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 6 seconds
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