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CYB5A Rabbit pAb

Anti-CYB5A Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217335

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217335
Product Name:   Anti-CYB5A Rabbit Polyclonal Antibody
Synonyms:   CYB5; MCB5
UNIPROT ID:   P00167 (Gene Accession – NP_683725 )
Background:    The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Fusion protein of human CYB5A
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 217335(CYB5A Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 217335(Anti-CYB5A Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 217335(Anti-CYB5A Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with fusion protein and then with D222195(Anti-CYB5A Antibody) at dilution 1/30.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Mouse kidney tissue, human fetal liver tissue, mouse liver tissue;
Primary antibody: 217335(CYB5A Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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