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CYB5D1 Rabbit pAb

Anti-CYB5D1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221864

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221864
Product Name:   Anti-CYB5D1 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   Q6P9G0 (Gene Accession – NP_653208 )
Background:    CYB5D1 belongs to the cytochrome b5 family and contains 1 cytochrome b5 heme-binding domain. Cytochromes b5 are ubiquitous electron transport proteins found in animals, plants and yeasts. There are 2 named isoforms produced by alternative splicing.
Immunogen:    Synthetic peptide of human CYB5D1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 20-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221864(CYB5D1 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221864(Anti-CYB5D1 Antibody) at dilution 1/20.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Hela, PC-3, A549, 293T and 231 cell lysates;
Primary antibody: 221864(CYB5D1 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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