Email: info@neweastbio.com   |  Call: 6109452007

CYB5R1 Rabbit pAb

Anti-CYB5R1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218560

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218560
Product Name:   Anti-CYB5R1 Rabbit Polyclonal Antibody
Synonyms:   B5R1; B5R2; B5R.1; NQO3A2; humb5R2
UNIPROT ID:   Q9UHQ9 (Gene Accession – BC018732 )
Background:    NADH-cytochrome b5 reductases participate in a variety of physiological processes including biosynthesis of cholesterol, methemoglobin reduction of erythrocytes, elongation of fatty acids and metabolism of drugs. CYB5R1 (cytochrome b5 reductase 1), also known as NADH-cytochrome b5 reductase 1, B5R1, NQO3A2, humb5R2 or NAD(P)H:quinone oxidoreductase type 3 polypeptide A2, is a 305 amino acid single-pass membrane protein that contains one FAD-binding FR-type domain and belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Widely expressed, CYB5R1 binds FAD as a cofactor and is encoded by a gene located on human chromosome 1. Human chromosome 1 spans 260 million base pairs, contains over 3,000 genes, comprises nearly 8% of the human genome and houses a large number of disease-associated genes, including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson’s disease, Gaucher disease, schizophrenia and Usher syndrome.
Immunogen:    Fusion protein of human CYB5R1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 218560(CYB5R1 Antibody) at a dilution of 1/30(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 218560(Anti-CYB5R1 Antibody) at dilution 1/30.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: K562, Hela and HEPG2 cell lysates;
Primary antibody: 218560(CYB5R1 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
          Publications