Email: info@neweastbio.com   |  Call: 6109452007

DDI2 Rabbit pAb

Anti-DDI2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218584

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218584
Product Name:   Anti-DDI2 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   Q5TDH0 (Gene Accession – BC006011 )
Background:    DDI1 and DDI2 are ubiquitin receptor homologs of the Saccharomyces cerevisiae ddi1 protein, which is involved in regulation of the cell cycle and the late secretory pathway. DDI2 is a 399 amino acid protein that contains one ubiquitin-like domain and exists as three isoforms as a result of alternative splicing. The gene encoding DDI2 maps to human chromosome 1, the largest human chromosome which spans about 260 million base pairs and makes up 8% of the human genome. Other notable genes located on chromosome 1 include LMNA, which is associated with the rare aging disease Hutchinson-Gilford progeria, and the MUTYH gene, which is partially responsible for familial adenomatous polyposis. Stickler syndrome, Parkinsons, Gaucher disease and Usher syndrome.
Immunogen:    Fusion protein of human DDI2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 218584(DDI2 Antibody) at a dilution of 1/75(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 218584(Anti-DDI2 Antibody) at dilution 1/75.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: HL60 and Jurkat cell lysates;
Primary antibody: 218584(DDI2 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
          Publications