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DDIT4 Rabbit pAb

Anti-DDIT4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217352

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217352
Product Name:   Anti-DDIT4 Rabbit Polyclonal Antibody
Synonyms:   Dig2; REDD1; REDD-1
UNIPROT ID:   Q9NX09 (Gene Accession – BC007714 )
Background:    Predicted to enable 14-3-3 protein binding activity. Involved in defense response to virus; negative regulation of TOR signaling; and response to hypoxia. Located in cytosol.
Immunogen:    Fusion protein of human DDIT4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cardiovascular, Cancer, Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 217352(DDIT4 Antibody) at a dilution of 1/100(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 217352(Anti-DDIT4 Antibody) at dilution 1/100.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: K562 and MCF7 cell lysates;
Primary antibody: 217352(DDIT4 Antibody) at dilution 1/1500;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 seconds
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