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DDX39B Rabbit pAb

Anti-DDX39B Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221071

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221071
Product Name:   Anti-DDX39B Rabbit Polyclonal Antibody
Synonyms:   BAT1; UAP56; D6S81E
UNIPROT ID:   Q13838 (Gene Accession – NP_004631 )
Background:    This gene encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and it also plays an important role in mRNA export from the nucleus to the cytoplasm. This gene belongs to a cluster of genes localized in the vicinity of the genes encoding tumor necrosis factor alpha and tumor necrosis factor beta.
Immunogen:    Synthetic peptide of human DDX39B
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 221071(DDX39B Antibody) at a dilution of 1/40(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the synthetic peptide and then with 221071(Anti-DDX39B Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 221071(Anti-DDX39B Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with synthetic peptide and then with D262439(Anti-DDX39B Antibody) at dilution 1/40.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: A431 cells, SKOV3 cells, 231 cells, Jurkat cells, HepG2 cells, Hela cells;
Primary antibody: 221071(DDX39B Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 second
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