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DDX43 Rabbit pAb

Anti-DDX43 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217355

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217355
Product Name:   Anti-DDX43 Rabbit Polyclonal Antibody
Synonyms:   CT13; HAGE
UNIPROT ID:   Q9NXZ2 (Gene Accession – BC066938 )
Background:    DEAD-box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome/spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis and cellular growth and division. DDX43 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 43), also known as CT13 or HAGE, is a 648 amino acid protein that contains one KH domain, one helicase C-terminal domain and one helicase ATP-binding domain and belongs to the DEAD-box family. Expressed in testis and present at abnormally high levels in a variety of tumors, DDX43 is thought to function as an ATP-dependent RNA helicase that may play a role tumor transformation and metastasis.
Immunogen:    Fusion protein of human DDX43
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 217355(DDX43 Antibody) at a dilution of 1/40(Cytoplasm or Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 217355(Anti-DDX43 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 217355(Anti-DDX43 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D222223(Anti-DDX43 Antibody) at dilution 1/40.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: HepG2 cells;
Primary antibody: 217355(DDX43 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 minutes
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