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DDX54 Rabbit pAb

Anti-DDX54 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218596

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218596
Product Name:   Anti-DDX54 Rabbit Polyclonal Antibody
Synonyms:   DP97
UNIPROT ID:   Q8TDD1 (Gene Accession – BC001132 )
Background:    This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The nucleolar protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors, and represses their transcriptional activity. Alternative splice variants that encode different isoforms have been found for this gene.
Immunogen:    Fusion protein of human DDX54
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: Oct-50;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 218596(DDX54 Antibody) at a dilution of 1/20(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 218596(Anti-DDX54 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: 293T, A549 and HEPG2 cell lysates;
Primary antibody: 218596(DDX54 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 second
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