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DDX56 Rabbit pAb

Anti-DDX56 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221885

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221885
Product Name:   Anti-DDX56 Rabbit Polyclonal Antibody
Synonyms:   DDX21; DDX26; NOH61
UNIPROT ID:   Q9NY93 (Gene Accession – NP_061955 )
Background:    This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. This gene may be involved in ribosome synthesis, most likely during assembly of the large 60S ribosomal subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Synthetic peptide of human DDX56
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 20-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 221885(DDX56 Antibody) at a dilution of 1/25(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the synthetic peptide and then with 221885(Anti-DDX56 Antibody) at dilution 1/25.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: HEPG2, 293T and Hela cell lysates;
Primary antibody: 221885(DDX56 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 7 seconds
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