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DDX59 Rabbit pAb

Anti-DDX59 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218597

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218597
Product Name:   Anti-DDX59 Rabbit Polyclonal Antibody
Synonyms:   OFD5; ZNHIT5
UNIPROT ID:   Q5T1V6 (Gene Accession – BC041801 )
Background:    DEAD-box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome/spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX59 (DEAD box protein 59), also known as ZNHIT5 (zinc finger HIT domaincontaining protein 5), is a 619 amino acid member of the DEAD box helicase protein family. Like many DEAD box helicase family members, DDX59 contains a Q motif, which controls ATP binding and hydrolysis. Expressed as two isoforms produced by alternative splicing, DDX59 contains one helicase C-terminal domain and one HIT-type zinc finger.
Immunogen:    Fusion protein of human DDX59
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 218597(DDX59 Antibody) at a dilution of 1/30(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 218597(Anti-DDX59 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: A549 and 293T cell lysates;
Primary antibody: 218597(DDX59 Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 7 minutes
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