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DEPTOR Rabbit pAb

Anti-DEPTOR Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219384

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219384
Product Name:   Anti-DEPTOR Rabbit Polyclonal Antibody
Synonyms:   DEP.6; DEPDC6
UNIPROT ID:   Q8TB45 (Gene Accession – BC024746 )
Background:    Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.
Immunogen:    Fusion protein of human DEPTOR
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 150-300;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 219384(DEPTOR Antibody) at a dilution of 1/120(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 219384(Anti-DEPTOR Antibody) at dilution 1/120.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 219384(Anti-DEPTOR Antibody) at a dilution of 1/120.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D226602(Anti-DEPTOR Antibody) at dilution 1/120.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: LOVO, 231 and 293T cell lysates;
Primary antibody: 219384(DEPTOR Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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