Email: info@neweastbio.com   |  Call: 6109452007

DEPTOR Rabbit pAb

Anti-DEPTOR Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221897

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221897
Product Name:   Anti-DEPTOR Rabbit Polyclonal Antibody
Synonyms:   DEP.6; DEPDC6
UNIPROT ID:   Q8TB45 (Gene Accession – NP_073620 )
Background:    DEPTOR (DEP domain containing MTOR-interacting protein), also known as DEP.6 or DEPDC6 (DEP domain-containing protein 6), is a 409 amino acid protein that negatively regulates mTORC1 and mTORC2 pathways. DEPTOR interacts with FRAP via its PDZ domain, and undergoes post-translational phosphorylation. Containing two DEP domains and one PDZ (DHR) domain, DEPTOR is encoded by a gene that maps to human chromosome 8q24.12. Chromosome 8 consists of nearly 146 million base pairs, encodes over 800 genes and is associated with a variety of diseases and malignancies. Schizophrenia, bipolar disorder, Trisomy 8, Pfeiffer syndrome, congenital hypothyroidism, Waardenburg syndrome and some leukemias and lymphomas are thought to occur as a result of defects in specific genes that map to chromosome 8.
Immunogen:    Synthetic peptide of human DEPTOR
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 20-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 221897(DEPTOR Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the synthetic peptide and then with 221897(Anti-DEPTOR Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse liver tissue lysate;
Primary antibody: 221897(DEPTOR Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
          Publications