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DGCR8 Rabbit mAb

Anti-DGCR8 Rabbit Monoclonal Antibody
5/5

$349.00

Cat.#:  N262131

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N262131
Product Name:   Anti-DGCR8 Rabbit Monoclonal Antibody
Synonyms:   DGCR8; DGCRK6; Gy1; C22orf12; D16Wis2; pasha
UNIPROT ID:   Q8WYQ5
Background:    Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs.
Immunogen:    Recombinant protein of human DGCR8
Applications:    WB,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R05-5A6
MW:    Calculated MW: 86 kDa; Observed MW: 120 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Western blot analysis of DGCR8 in K562, C6, 3T3 lysates using DGCR8 antibody.

Immunocytochemistry analysis of DGCR8 (green) in K562 using DGCR8 antibody,and DAPI(blue).
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