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DLK1 Rabbit pAb

Anti-DLK1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216473

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216473
Product Name:   Anti-DLK1 Rabbit Polyclonal Antibody
Synonyms:   DLK; FA1; ZOG; pG2; DLK-1; PREF1; Delta1; Pref-1
UNIPROT ID:   P80370 (Gene Accession – BC007741 )
Background:    This gene encodes a transmembrane protein that contains multiple epidermal growth factor repeats that functions as a regulator of cell growth. The encoded protein is involved in the differentiation of several cell types including adipocytes. This gene is located in a region of chromosome 14 frequently showing unparental disomy, and is imprinted and expressed from the paternal allele. A single nucleotide variant in this gene is associated with child and adolescent obesity and shows polar overdominance, where heterozygotes carrying an active paternal allele express the phenotype, while mutant homozygotes are normal.
Immunogen:    Fusion protein of human DLK1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Neuroscience, Stem Cells
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 216473(DLK1 Antibody) at a dilution of 1/30(Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the fusion protein and then with 216473(Anti-DLK1 Antibody) at dilution 1/30.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: HEPG2 and 231 cell lysates;
Primary antibody: 216473(DLK1 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 seconds
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