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DMAP1 Rabbit pAb

Anti-DMAP1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221910

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221910
Product Name:   Anti-DMAP1 Rabbit Polyclonal Antibody
Synonyms:   EAF2; SWC4; MEAF2; DNMAP1; DNMTAP1
UNIPROT ID:   Q9NPF5 (Gene Accession – EAX07050 )
Background:    This gene encodes a subunit of several, distinct complexes involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this comple,x providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. Alternatively spliced transcript variants encoding the same protein have been described.
Immunogen:    Synthetic peptide of human DMAP1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221910(DMAP1 Antibody) at a dilution of 1/25(Cytoplasm or Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221910(Anti-DMAP1 Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 221910(Anti-DMAP1 Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with synthetic peptide and then with D263722(Anti-DMAP1 Antibody) at dilution 1/25.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: RAW and NIH/3T3 cell lysates;
Primary antibody: 221910(DMAP1 Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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