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DOK2 Rabbit pAb

Anti-DOK2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221414

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221414
Product Name:   Anti-DOK2 Rabbit Polyclonal Antibody
Synonyms:   p56DOK; p56dok-2
UNIPROT ID:   O60496 (Gene Accession – NP_003965 )
Background:    The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells.
Immunogen:    Synthetic peptide of human DOK2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-50;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 221414(DOK2 Antibody) at a dilution of 1/35(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 221414(Anti-DOK2 Antibody) at dilution 1/35.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Raji cell lysate;
Primary antibody: 221414(DOK2 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 minutes
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