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E2F1 Rabbit pAb

Anti-E2F1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221227

   Size:   100 μl

In Stock

            Product Description          
Cat.#:   S221227
Product Name:   Anti-E2F1 Rabbit Polyclonal Antibody
Synonyms:   RBP3; E2F-1; RBAP1; RBBP3
UNIPROT ID:   Q01094 (Gene Accession – NP_005216 )
Background:    The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Immunogen:    Synthetic peptide of human E2F1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 221227(E2F1 Antibody) at a dilution of 1/50(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the synthetic peptide and then with 221227(Anti-E2F1 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 221227(Anti-E2F1 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with synthetic peptide and then with D262733(Anti-E2F1 Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: MCF-7, LNCAP, HepG2, Jurkat, Hela cell lysates;
Primary antibody: 221227(E2F1 Antibody) at dilution 1/1200;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 3 minutes
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