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E2F6 Rabbit pAb

Anti-E2F6 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S210287

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S210287
Product Name:   Anti-E2F6 Rabbit Polyclonal Antibody
Synonyms:   E2F-6
UNIPROT ID:   O75461 (Gene Accession – BC008348 )
Background:    This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants.
Immunogen:    Fusion protein of human E2F6
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 210287(E2F6 Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 210287(Anti-E2F6 Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 210287(Anti-E2F6 Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D120574(Anti-E2F6 Antibody) at dilution 1/60.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: LoVo cells, A549 cells, human hepatocellular carcinoma tissue, Jurkat cells, Hela cells;
Primary antibody: 210287(E2F6 Antibody) at dilution 1/650;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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