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ECHS1 Rabbit pAb

Anti-ECHS1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217381

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217381
Product Name:   Anti-ECHS1 Rabbit Polyclonal Antibody
Synonyms:   SCEH; mECH; mECH1; ECHS1D
UNIPROT ID:   P30084 (Gene Accession – BC008906 )
Background:    The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature.
Immunogen:    Fusion protein of human ECHS1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 217381(ECHS1 Antibody) at a dilution of 1/80(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 217381(Anti-ECHS1 Antibody) at dilution 1/80.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 217381(Anti-ECHS1 Antibody) at a dilution of 1/80.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D222269(Anti-ECHS1 Antibody) at dilution 1/80.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: HepG2, LO2, Mouse fetal liver tissue, Mouse liver tissue, Hela, PC3, MCF7 cell lysates;
Primary antibody: 217381(ECHS1 Antibody) at dilution 1/1300;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds
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