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ECSIT Rabbit pAb

Anti-ECSIT Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219120

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219120
Product Name:   Anti-ECSIT Rabbit Polyclonal Antibody
Synonyms:   SITPEC
UNIPROT ID:   Q9BQ95 (Gene Accession – BC000193 )
Background:    Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development (By similarity).
Immunogen:    Fusion protein of human ECSIT
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-300;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 219120(ECSIT Antibody) at a dilution of 1/70(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 219120(Anti-ECSIT Antibody) at dilution 1/70.

The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 219120(Anti-ECSIT Antibody) at a dilution of 1/70.

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with fusion protein and then with D225876(Anti-ECSIT Antibody) at dilution 1/70.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Jurkat and HepG2 cell lysates;
Primary antibody: 219120(ECSIT Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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