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EDA2R Rabbit pAb

Anti-EDA2R Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217384

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217384
Product Name:   Anti-EDA2R Rabbit Polyclonal Antibody
Synonyms:   XEDAR; EDAA2R; EDA-A2R; TNFRSF27
UNIPROT ID:   Q9HAV5 (Gene Accession – BC034919 )
Background:    EDA-A1 and EDA-A2 are two isoforms of ectodysplasin that are encoded by the anhidrotic ectodermal dysplasia (EDA) gene. Mutations in EDA give rise to a clinical syndrome characterized by loss of hair, sweat glands, and teeth. The protein encoded by this gene specifically binds to EDA-A2 isoform. This protein is a type III transmembrane protein of the TNFR (tumor necrosis factor receptor) superfamily, and contains 3 cysteine-rich repeats and a single transmembrane domain but lacks an N-terminal signal peptide. Alternatively spliced transcript variants have been found for this gene.
Immunogen:    Fusion protein of human EDA2R
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 217384(EDA2R Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 217384(Anti-EDA2R Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 217384(Anti-EDA2R Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with fusion protein and then with D222272(Anti-EDA2R Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: 293T cells;
Primary antibody: 217384(EDA2R Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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