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EDNRA Rabbit pAb

Anti-EDNRA Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219755

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219755
Product Name:   Anti-EDNRA Rabbit Polyclonal Antibody
Synonyms:   ETA; ET-A; ETAR; ETRA; MFDA; ETA-R; hET-AR
UNIPROT ID:   P25101 (Gene Accession – NP_001948 )
Background:    This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants.
Immunogen:    Synthetic peptide of human EDNRA
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer, Cardiovascular, Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 219755(EDNRA Antibody) at a dilution of 1/50(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 219755(Anti-EDNRA Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 219755(Anti-EDNRA Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with synthetic peptide and then with D260290(Anti-EDNRA Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: A549 cell lysate;
Primary antibody: 219755(EDNRA Antibody) at dilution 1/500;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 minutes
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