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EEA1 Rabbit pAb

Anti-EEA1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S215698

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S215698
Product Name:   Anti-EEA1 Rabbit Polyclonal Antibody
Synonyms:   MST105; ZFYVE2; MSTP105
UNIPROT ID:   Q15075 (Gene Accession – NP_003557 )
Background:    Enables 1-phosphatidylinositol binding activity; GTP-dependent protein binding activity; and protein homodimerization activity. Involved in endocytosis; vesicle fusion; and viral RNA genome replication. Located in cytosol and early endosome. Is extrinsic component of plasma membrane.
Immunogen:    Synthetic peptide of human EEA1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 215698(EEA1 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 215698(Anti-EEA1 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 215698(Anti-EEA1 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with synthetic peptide and then with D163757(Anti-EEA1 Antibody) at dilution 1/50.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: 293T, Hela, HepG2 cell lysates;
Primary antibody: 215698(EEA1 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 90 seconds
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