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EGFR (5E10) Mouse mAb

Anti-EGFR (5E10) Mouse Monoclonal Antibody
5/5

$349.00

Cat.#:  N261441

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N261441
Product Name:   Anti-EGFR (5E10) Mouse Monoclonal Antibody
Synonyms:   EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
UNIPROT ID:   P00533
Background:    EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. . A single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation.
Immunogen:    Purified recombinant human EGFR protein fragments expressed in E.coli.
Applications:    WB,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    5E10-G10-E11
MW:    Calculated MW: 134 kDa; Observed MW: 175 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human,Monkey
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of EGFR (5E10) in HeLa using EGFR antibody.

Western blot analysis of EGFR in A549, MDA-MB-468 and COS7 lysates using EGFR antibody.

Immunoprecipitation analysis of EGFR (5E10) in Hela lysates using EGFR antibody.

Immunocytochemistry analysis of EGFR in MDA-MB-468 cells using EGFR antibody.
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