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EGR4 Rabbit pAb

Anti-EGR4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219757

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219757
Product Name:   Anti-EGR4 Rabbit Polyclonal Antibody
Synonyms:   NGFIC, NGFI-C, PAT133
UNIPROT ID:   Q05215 (Gene Accession – NP_001956 )
Background:    Early growth response protein 4 (EGR-4), also known as AT133, is a protein that in humans is encoded by the EGR4 gene. EGR-4 is a member of the early growth response (EGF) family of zinc finger transcription factors. Transcriptional regulator. Recognizes and binds to the DNA sequence 5′-GCGGGGGCG-3′ (GSG). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
Immunogen:    Synthetic peptide of human EGR4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 219757(EGR4 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 219757(Anti-EGR4 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 219757(Anti-EGR4 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D260293(Anti-EGR4 Antibody) at dilution 1/20.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Human fetal brain tissue, Mouse brain tissue;
Primary antibody: 219757(EGR4 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes
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