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EIF2AK3 Rabbit pAb

Anti-EIF2AK3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216724

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216724
Product Name:   Anti-EIF2AK3 Rabbit Polyclonal Antibody
Synonyms:   PEK; WRS; PERK
UNIPROT ID:   Q9NZJ5 (Gene Accession – BC126354 )
Background:    The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome.
Immunogen:    Fusion protein of human EIF2AK3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 216724(EIF2AK3 Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 216724(Anti-EIF2AK3 Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 216724(Anti-EIF2AK3 Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with fusion protein and then with D221128(Anti-EIF2AK3 Antibody) at dilution 1/60.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: HepG2 cell lysate;
Primary antibody: 216724(EIF2AK3 Antibody) at dilution 1/400;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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