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EIF3F Rabbit pAb

Anti-EIF3F Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217391

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217391
Product Name:   Anti-EIF3F Rabbit Polyclonal Antibody
Synonyms:   EIF3S5; eIF3-p47
UNIPROT ID:   O00303 (Gene Accession – BC000490 )
Background:    Eukaryotic translation initiation factor 3 subunit F is a protein that in humans is encoded by the EIF3F gene. The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Immunogen:    Fusion protein of human EIF3F
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 217391(EIF3F Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 217391(Anti-EIF3F Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 217391(Anti-EIF3F Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D222281(Anti-EIF3F Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse skin tissue;
Primary antibody: 217391(EIF3F Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds
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