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eIF4E Rabbit mAb

Anti-eIF4E Rabbit Monoclonal Antibody
5/5

$349.00

Cat.#:  N262170

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N262170
Product Name:   Anti-eIF4E Rabbit Monoclonal Antibody
Synonyms:   EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein
UNIPROT ID:   P06730
Background:    eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.
Immunogen:    A synthetic peptide of human eIF4E
Applications:    WB,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R08-1C5
MW:    Calculated MW: 25 kDa; Observed MW: 25 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Hypoxia Signal Transductionyyyyy Hypoxia-induced
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Western blot analysis of eIF4E in C6, 3T3 lysates using eIF4E antibody

Immunocytochemistry analysis of eIF4E (green) in HCT116 using eIF4E antibody,and DAPI(blue).

Western blot analysis of eIF4E in HL-60 lysates using eIF4E antibody.
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