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eIF4G1 Rabbit pAb

Anti-eIF4G1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225280

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225280
Product Name:   Anti-eIF4G1 Rabbit pAb
Synonyms:   p220
UNIPROT ID:   Q04637
Background:    Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5′-terminal secondary structure and recruitment of mRNA to the ribosome.
Immunogen:    Peptide sequence around aa.1230~1234 (P-V-S-P-L) derived from Human eIF4G.
Applications:    WB,IHC-P,ICC/IF
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 175 kDa; Observed MW: 220 kDa
Isotype:    IgG
Purification:    Affinity Chromatography
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunofluorescence analysis of eIF4G1 in Hela using eIF4G (Ab1232) antibody.

Western blot analysis of eIF4G1 in HepG2 and HT-29 lysates using eIF4G (Ab1232) antibody.

Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma tissue using eIF4G (Ab1232) antibody (left) or the same antibody preincubated with blocking peptide (right).High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of eIF4G1 in Hela and MCF-7 lysates using eIF4G1 antibody
          Publications