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ELAVL3 Rabbit pAb

Anti-ELAVL3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220658

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220658
Product Name:   Anti-ELAVL3 Rabbit Polyclonal Antibody
Synonyms:   HUC; HUCL; PLE21
UNIPROT ID:   Q14576 (Gene Accession – NP_001411 )
Background:    A member of the ELAVL protein family, ELAV-like 3 is a neural-specific RNA-binding protein which contains three RNP-type RNA recognition motifs. The observation that ELAVL3 is one of several Hu antigens (neuronal-specific RNA-binding proteins) recognized by the anti-Hu serum antibody present in sera from patients with paraneoplastic encephalomyelitis and sensory neuronopathy (PEM/PSN) suggests it has a role in neurogenesis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Immunogen:    Synthetic peptide of human ELAVL3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 220658(ELAVL3 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the synthetic peptide and then with 220658(Anti-ELAVL3 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 220658(Anti-ELAVL3 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D261834(Anti-ELAVL3 Antibody) at dilution 1/20.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane: Human fetal brain tissue;
Primary antibody: 220658(ELAVL3 Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 minutes
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