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EN1 Rabbit pAb

Anti-EN1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221953

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221953
Product Name:   Anti-EN1 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   Q05925 (Gene Accession – NP_001417 )
Background:    Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the ‘engrailed’ (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.
Immunogen:    Synthetic peptide of human EN1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 20-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Neuroscience, Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 221953(EN1 Antibody) at a dilution of 1/20(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the synthetic peptide and then with 221953(Anti-EN1 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: A172 and HEPG2 cell lysates;
Primary antibody: 221953(EN1 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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