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ENTPD1 (CD39) Rabbit pAb

Anti-ENTPD1 (CD39) Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221122

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221122
Product Name:   Anti-ENTPD1 (CD39) Rabbit Polyclonal Antibody
Synonyms:   CD39; SPG64; ATPDase; NTPDase-1
Target:    CD39/ENTPD1 (ectonucleoside triphosphate diphosphohydrolase 1)
UNIPROT ID:   P49961 (Gene Accession – NP_001767 )
Background:    CD39/ENTPD1 is the major ectonucleotidase expressed in macrophages. It hydrolyzes extracellular ATP and ADP to AMP. This enzyme plays an important immunomodulatory role on macrophages by regulating the extracellular ATP levels and limiting P2-mediated cellular responses, such as apoptosis, NLRP3 inflammasome activation, and IL-1β release. In neutrophil, impairment of nucleotide catabolism by pharmacologic inhibition or genetic deletion of CD39 increases the activation, chemotaxis, and adhesion of neutrophils to the vascular endothelium. In natural killer (NK) cells, a type of cytotoxic lymphocyte, which are also crucial to the innate immune responses, it is the dominant ectonucleotidase. CD39 activity in NK cells augments the capacity of these cells to secrete IFNγ by modulating P2 receptor activation. Thus, CD39 deletion on NK cells provides end-organ protection decreasing tissue damage mediated by NK cells. Finally, in dendritic cells (DCs) that are cells classically responsible for linking innate to adaptive immunity by capturing, processing, presenting antigens, and migrating to lymphoid tissues for the stimulation of antigen-reactive T cells, CD39 activity can regulate nucleotide levels, consequently, impair DCs’ responses.
Immunogen:    Synthetic peptide of human ENTPD1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing
Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 221122(ENTPD1 Antibody) at a dilution of 1/50(Cytoplasm). In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the synthetic peptide and then with 221122(Anti-ENTPD1 Antibody) at dilution 1/50.
Gel: 8%SDS-PAGE, Lysate: 40 μg; Lane 1-2: Hela and Jurkat cell lysates; Primary antibody: 221122(ENTPD1 Antibody) at dilution 1/1200; Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution; Exposure time: 1 minute
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