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EPCAM Rabbit pAb

Anti-EPCAM Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219325

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219325
Product Name:   Anti-EPCAM Rabbit Polyclonal Antibody
Synonyms:   ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
UNIPROT ID:   P16422 (Gene Accession – BC014785 )
Background:    This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
Immunogen:    Fusion protein of human EPCAM
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 219325(EPCAM Antibody) at a dilution of 1/60(Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 219325(Anti-EPCAM Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using 219325(Anti-EPCAM Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with fusion protein and then with D226394(Anti-EPCAM Antibody) at dilution 1/60.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: HT-29 cell lysate;
Primary antibody: 219325(EPCAM Antibody) at dilution 1/900;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
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