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ETFB Rabbit pAb

Anti-ETFB Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220533

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220533
Product Name:   Anti-ETFB Rabbit Polyclonal Antibody
Synonyms:   MADD; FP585
UNIPROT ID:   P38117 (Gene Accession – NP_001976 )
Background:    This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene.
Immunogen:    Synthetic peptide of human ETFB
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gasrtic cancer tissue using 220533(ETFB Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human gasrtic cancer tissue is first treated with the synthetic peptide and then with 220533(Anti-ETFB Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 220533(Anti-ETFB Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D261662(Anti-ETFB Antibody) at dilution 1/25.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: HepG2 cells, A549 cells, Hela cells, human fetal liver tissue, mouse liver tissue;
Primary antibody: 220533(ETFB Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds
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