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FAK Rabbit pAb

Anti-FAK Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225445

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225445
Product Name:   Anti-FAK Rabbit pAb
Synonyms:   PTK2; FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK
UNIPROT ID:   Q05397
Background:    This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix.
Immunogen:    The antiserum was produced against synthesized peptide derived from human FAK. AA range:373-422
Applications:    WB,IHC-P,ELISA
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 119 kDa; Observed MW: 119 kDa
Isotype:    IgG
Purification:    Affinity Chromatography
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using FAK antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Sample with blocking peptide on the right.

Western blot analysis of FAK in various lysates using FAK antibody.

Western blot analysis of FAK in HT-29 lysates using FAK antibody.

Western blot analysis of FAK in HeLa lysates treated with EGF using FAK antibody.The lane on the right is blocked with the synthesized peptide.
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