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FGF2 Rabbit pAb

Anti-FGF2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219671

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219671
Product Name:   Anti-FGF2 Rabbit Polyclonal Antibody
Synonyms:   BFGF; FGFB; FGF-2; HBGF-2
UNIPROT ID:   P09038 (Gene Accession – NP_001997 )
Background:    The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
Immunogen:    Synthetic peptide of human FGF2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cardiovascular, Cancer, Neuroscience, Stem Cells, Developmental Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 219671(FGF2 Antibody) at a dilution of 1/50(Secreted).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the synthetic peptide and then with 219671(Anti-FGF2 Antibody) at dilution 1/50.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Hela cell, HepG2 cell, LOVO cell lysates;
Primary antibody: 219671(FGF2 Antibody) at dilution 1/400;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 15 seconds
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