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FHIT Rabbit pAb

Anti-FHIT Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216517

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216517
Product Name:   Anti-FHIT Rabbit Polyclonal Antibody
Synonyms:   FRA3B, AP3Aase
UNIPROT ID:   P49789 (Gene Accession – BC032336 )
Background:    This gene, a member of the histidine triad gene family, encodes a diadenosine 5′,5′-P1,P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for this gene.
Immunogen:    Fusion protein of human FHIT
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 216517(FHIT Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 216517(Anti-FHIT Antibody) at dilution 1/25.

Gel: 8+12%SDS-PAGE, Lysate: 30 μg;
Lane: Human fetal kidney tissue;
Primary antibody: 216517(FHIT Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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