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FRMD5 Rabbit pAb

Anti-FRMD5 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219432

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219432
Product Name:   Anti-FRMD5 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   Q7Z6J6 (Gene Accession – BC053647 )
Background:    May be involved in regulation of cell migration (PubMed:22846708, PubMed:25448675). May regulate cell-matrix interactions via its interaction with ITGB5 and modifying ITGB5 cytoplasmic tail interactions such as with FERMT2 and TLN1. May regulate ROCK1 kinase activity possibly involved in regulation of actin stress fiber formation (PubMed:25448675).
Immunogen:    Fusion protein of human FRMD5
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 150-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 219432(FRMD5 Antibody) at a dilution of 1/160(Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 219432(Anti-FRMD5 Antibody) at dilution 1/160.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: HUVEC cell, Mouse kidney tissue lysates;
Primary antibody: 219432(FRMD5 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 3 minutes
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