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GAB4 Rabbit pAb

Anti-GAB4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S222056

   Size:   100 μl

In Stock

            Product Description          
Cat.#:   S222056
Product Name:   Anti-GAB4 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   Q2WGN9 (Gene Accession – NP_001032903 )
Background:    The function of this protein remains unknown.
Immunogen:    Synthetic peptide of human GAB4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-50;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 222056(GAB4 Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the synthetic peptide and then with 222056(Anti-GAB4 Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 222056(Anti-GAB4 Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with synthetic peptide and then with D263954(Anti-GAB4 Antibody) at dilution 1/25.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: SKOV3 and LOVO cell lysates;
Primary antibody: 222056(GAB4 Antibody) at dilution 1/650;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 minutes
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