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GAPDHS Rabbit pAb

Anti-GAPDHS Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  22079

   Size:   100 μl

In Stock

          Product Description          
Cat. #:   22079
Product Name:   Anti-GAPDHS Rabbit Polyclonal Antibody
Synonyms:   GAPD2; GAPDS; HSD-35; GAPDH-2; HEL-S-278
UNIPROT ID:   O14556 (Gene Accession – NP_055179 )
Background:    This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility.
Immunogen:    Synthetic peptide of human GAPDHS
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing
Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 214127(GAPDHS Antibody) at a dilution of 1/50(Cytoplasm). In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 214127(Anti-GAPDHS Antibody) at dilution 1/50.
Gel: 8%SDS-PAGE, Lysate: 40 μg; Lane: A549 cell lysate; Primary antibody: 214127(GAPDHS Antibody) at dilution 1/400; Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution; Exposure time: 2 minutes
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