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GARS1 Rabbit pAb

Anti-GARS1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S212206

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S212206
Product Name:   Anti-GARS1 Rabbit Polyclonal Antibody
Synonyms:   GARS; HMN5; CMT2D; DSMAV; GlyRS; SMAD1
UNIPROT ID:   P41250 (Gene Accession – BC000065 )
Background:    This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]
Immunogen:    Fusion protein of human GARS1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 212206(GARS1 Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the fusion protein and then with 212206(Anti-GARS1 Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 212206(Anti-GARS1 Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with fusion protein and then with D124523(Anti-GARS1 Antibody) at dilution 1/60.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: 293T,Jurkat,Raji and HT-29 cell?Mouse brain tissue?A431 and Hela cell lysates;
Primary antibody: 212206(GARS1 Antibody) at dilution 1/800;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 60 seconds
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