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GKN1 Rabbit pAb

Anti-GKN1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221422

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221422
Product Name:   Anti-GKN1 Rabbit Polyclonal Antibody
Synonyms:   FOV; CA11; AMP18; BRICD1; foveolin
UNIPROT ID:   Q9NS71 (Gene Accession – NP_062563 )
Background:    Gastrokine 1 (GKN1), a stomach-speci?c protein also known as 18 kDa antrum mucosa protein (AMP-18) or foveolin, belongs to the gastrokine family of gastric mucus cell-secreted proteins. The human GKN1 gene has been localized in a region of chromosome 2p13 of about 6 kb and contains 6 exons. GKN1 is expressed only in normal human stomach, but is absent from gastric adenocarcinomas, gastro-esophageal adenocarcinoma cell lines, and other normal and tumor gastro-intestinal tissues. GKN1may play an important role in normal gastric function and may be a gastric tumour suppressor.
Immunogen:    Synthetic peptide of human GKN1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 221422(GKN1 Antibody) at a dilution of 1/30(Secreted).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 221422(Anti-GKN1 Antibody) at dilution 1/30.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Human stomach tissue lysate;
Primary antibody: 221422(GKN1 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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