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GOLGA3 Rabbit pAb

Anti-GOLGA3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S222091

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S222091
Product Name:   Anti-GOLGA3 Rabbit Polyclonal Antibody
Synonyms:   MEA-2; GCP170
UNIPROT ID:   Q08378 (Gene Accession – NP_005886 )
Background:    The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes a member of the golgin family of proteins which are localized to the Golgi. Its encoded protein has been postulated to play a role in nuclear transport and Golgi apparatus localization. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.
Immunogen:    Synthetic peptide of human GOLGA3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 222091(GOLGA3 Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 222091(Anti-GOLGA3 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 222091(Anti-GOLGA3 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with synthetic peptide and then with D264008(Anti-GOLGA3 Antibody) at dilution 1/30.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: LO2 and 293T cell lysates;
Primary antibody: 222091(GOLGA3 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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