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GPI Rabbit pAb

Anti-GPI Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S215843

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S215843
Product Name:   Anti-GPI Rabbit Polyclonal Antibody
Synonyms:   AMF; NLK; PGI; PHI; GNPI; SA36; SA-36
UNIPROT ID:   P06744 (Gene Accession – NP_000166 )
Background:    This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants.
Immunogen:    Synthetic peptide of human GPI
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer, Neuroscience, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer using 215843(GPI Antibody) at a dilution of 1/45(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer is first treated with the synthetic peptide and then with 215843(Anti-GPI Antibody) at dilution 1/45.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer using 215843(Anti-GPI Antibody) at a dilution of 1/45.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer is first treated with synthetic peptide and then with D163998(Anti-GPI Antibody) at dilution 1/45.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: PC3, Raji, Hela, 231 and Jurkat cell lysates;
Primary antibody: 215843(GPI Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 3 minutes
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